Development of a pan-serotype reverse transcription loop-mediated isothermal amplification assay for the detection of dengue virus
نویسندگان
چکیده
منابع مشابه
Reverse transcription-loop-mediated isothermal amplification assay for rapid detection of hepatitis E virus.
The one-step single-tube betaine-free reverse transcription (RT)-loop-mediated isothermal amplification assay was developed for rapid diagnosis of hepatitis E virus. This assay amplified the target gene in less than 45 min (even as short as 20 min) under isothermal conditions at 63 degrees C, and the sensitivity of this assay was 100-fold greater than that of RT-PCR. This assay demonstrated a d...
متن کاملRapid detection and differentiation of dengue virus serotypes by a real-time reverse transcription-loop-mediated isothermal amplification assay.
The development and validation of a one-step, real-time, and quantitative dengue virus serotype-specific reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay targeting the 3' noncoding region for the rapid detection and differentiation of dengue virus serotypes are reported. The RT-LAMP assay is very simple and rapid, wherein the amplification can be obtained in 30 min u...
متن کاملA reverse transcriptase-loop mediated isothermal amplification assay (RT-LAMP) for rapid detection of bovine viral diarrhea virus 1 and 2
Bovine viral diarrhea virus (BVDV) is a pathogen that infects cattle, and is globally important. It causes substantial financial losses to the livestock industry. In the current study, a one-step reverse transcriptase-loop-mediated isothermal amplification (RT-LAMP) assay was set up for rapid and efficient detection of BVDV. For this purpose, four primers were designed to recognize six distinct...
متن کاملAccuracy of Reverse Transcription Loop-Mediated Isothermal Amplification Technique for Detecting Dengue Virus
In recent years, researchers have developed a reverse transcription loop-mediated isothermal amplification (RT-LAMP) test as a sensitive and specific technique. But the results of these studies were conflicting. The aim of this meta-analysis was to assess the accuracy of RT-LAMP technique for detecting dengue virus. We systematically searched PUBMED, EMBASE, Web of Science, and the Cochrane Lib...
متن کاملReverse transcription loop-mediated isothermal amplification assay for rapid detection of Bovine Rotavirus
BACKGROUND Bovine rotavirus (BRV) infection is common in young calves. This viral infection causes acute diarrhea leading to death. Rapid identification of infected calves is essential to control BRV successfully. Therefore development of simple, highly specific, and sensitive detection method for BRV is needed. RESULTS A reverse transcription loop-mediated isothermal amplification (RT-LAMP) ...
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ژورنال
عنوان ژورنال: Diagnostic Microbiology and Infectious Disease
سال: 2015
ISSN: 0732-8893
DOI: 10.1016/j.diagmicrobio.2015.05.004